TABLE 6.

The bre1 mutation decreases GCR rates from a subset of GCR mutator strains

Relevant genotypeaWild typebre1Δ
Strain numberGCR rate (CANr-5FOAr)bStrain numberGCR rate (CANr-5FOAr)b
Wild typeRDKY36153.5 × 10−10 (1)YKJM2233>9.9 × 10−10 (3)
rad5ΔYKJM13852.4 × 10−8 (68)YKJM26267.4 × 10−10 (2)
rad18ΔYKJM13892.3 × 10−8 (65)YKJM2628>2.6 × 10−9 (7)
mec1Δ sml1ΔRDKY37354.6 × 10−8 (131)YKJM23777.4 × 10−9 (21)
pif1-m2RDKY43434.8 × 10−8 (137)YKJM22911.0 × 10−8 (29)
  • a All strains are isogenic with the wild-type strain RDKY3615 (MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 hxt13::URA3) with the exception of the indicated mutations. The GCR rates of YKJM2282 (rad6 rad5) and YKJM2284 (rad6 rad18) are 2.9 × 10−9 (8) and 1.7 × 10−9 (5), respectively. Parentheses indicate the rate relative to wild type.

  • b CANr-5FOAr, canavanine and 5FOA resistant.