TABLE 5.

The siz1 mutation decreases GCR rates from a subset of GCR mutator strains

Relevant genotypeaWild typesiz1Δ
Strain numberGCR rate (CANr-5FOAr)bStrain numberGCR rate (CANr-5FOAr)b
Wild typeRDKY36153.5 × 10−10 (1)YKJM21791.3 × 10−9 (4)
rad5ΔYKJM13852.4 × 10−8 (68)YKJM21821.1 × 10−9 (3)
rad18ΔYKJM13892.3 × 10−8 (65)YKJM2185>1.2 × 10−9 (4)
mec1Δ sml1ΔRDKY37354.6 × 10−8 (131)YKJM29591.1 × 10−9 (3)
rfa1-t33RDKY36172.7 × 10−7 (771)YKJM29611.3 × 10−7 (377)
rad27ΔRDKY36303.4 × 10−7 (971)YKJM29574.3 × 10−7 (1,229)
mre11ΔRDKY36332.2 × 10−7 (629)YKJM29631.8 × 10−8 (514)
pif1-m2RDKY43434.8 × 10−8 (137)YKJM22273.7 × 10−8 (107)
  • a All strains are isogenic with the wild-type strain RDKY3615 (MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 hxt13::URA3) with the exception of the indicated mutations. The GCR rate of YKJM2368 (rad5 rad18 siz1) is 5.6 × 10−9 (16). Parentheses indicate the rate relative to wild type.

  • b CANr-5FOAr, canavanine and 5FOA resistant.