TABLE 4.

GCR formation enhanced by rad5 or rad18 mutation is decreased by an inactivation affecting homologous recombination

Relevant genotypeaWild typerad5Δrad18Δ
Strain numberGCR rate (CANr-5FOAr)bStrain numberGCR rate (CANr-5FOAr)bStrain numberGCR rate (CANr-5FOAr)b
Wild typeRDKY36153.5 × 10−10 (1)YKJM13852.4 × 10−8 (68)YKJM13892.3 × 10−8 (65)
rad51ΔRDKY36363.5 × 10−9 (10)YKJM1883>2.2 × 10−9 (6)YKJM1901>1.8 × 10−9 (5)
rad52ΔRDKY44213.5 × 10−8 (100)YKJM1903>2.4 × 10−9 (7)YKJM1905>3.1 × 10−9 (9)
rad54ΔRDKY44731.9 × 10−9 (5)YKJM20252.6 × 10−9 (8)YKJM20331.7 × 10−9 (5)
rad55ΔRDKY52031.9 × 10−9 (5)YKJM2594>3.0 × 10−9 (9)YKJM2596>3.0 × 10−9 (9)
rad59ΔRDKY44237.5 × 10−9 (21)YKJM20295.6 × 10−8 (159)YKJM20375.0 × 10−8 (142)
srs2ΔYKJM0315>3.1 × 10−10 (0.9)YKJM1907>3.1 × 10−10 (0.9)YKJM1909>2.9 × 10−10 (0.8)
srs2-K41RYKJM3034>1.9 × 10−9 (5)YKJM3037>1.2 × 10−9 (3)YKJM3038>1.1 × 10−9 (3)
pol30-119YKJM26241.0 × 10−9 (3)YKJM32098.3 × 10−10 (2)YKJM27428.4 × 10−10 (2)
  • a All strains are isogenic with the wild-type strain RDKY3615 (MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 hxt13::URA3) with the exception of the indicated mutations. The GCR rate of YKJM3905 (rad18 srs2 rad51) is 1.0 × 10−8 (28). Parentheses indicate the rate relative to wild type.

  • b CANr-5FOAr, canavanine and 5FOA resistant..