TABLE 2.

Defects in proteins functioning in PRR caused different effects in the rate of GCR formation

Relevant genotypeaWild typepif1-m2
Strain numberGCR rate (CANr-5FOAr)bStrain numberGCR rate (CANr-5FOAr)b
Wild typeRDKY36153.5 × 10−10 (1)RDKY43434.8 × 10−8 (137)
rad5ΔYKJM13852.4 × 10−8 (68)YKJM13872.2 × 10−7 (629)
rad18ΔYKJM13892.3 × 10−8 (65)YKJM13912.5 × 10−7 (714)
rev1ΔYKJM21688.3 × 10−10 (2)YKJM15886.5 × 10−8 (185)
rev3ΔYKJM15586.4 × 10−10 (2)YKJM15695.0 × 10−8 (143)
rev7ΔYKJM2170>1.2 × 10−9 (3)YKJM22314.5 × 10−8 (130)
rad30ΔYKJM1215.8 × 10−10 (2)YKJM15523.7 × 10−8 (106)
pol32ΔYKJM15606.5 × 10−10 (2)YKJM15676.8 × 10−8 (194)
ubc13ΔYKJM15851.3 × 10−9 (4)YKJM15794.6 × 10−8 (130)
mms2ΔYKJM2135>2.6 × 10−10 (1)YKJM22293.4 × 10−8 (98)
rad6ΔYKJM15736.1 × 10−10 (2)YKJM15662.3 × 10−8 (66)
  • a All strains are isogenic with the wild-type strain RDKY3615 (MATa ura3-52 leu2Δ1 trp1Δ63 his3Δ200 lys2ΔBgl hom3-10 ade2Δ1 ade8 hxt13::URA3) with the exception of the indicated mutations. The GCR rates of YKJM1519 (rad5 rad18), YKJM3240 (rev1 rev3 rad30), YKJM2176 (rev3 rev7 rad30), and YKJM3236 (rev3 rad30 pol32) are 2.9 × 10−8 (83), >3.9 × 10−9 (4), 5.8 × 10−10 (2), and >1.7 × 10−9 (3), respectively. Parentheses indicate the rate relative to wild type.

  • b CANr-5FOAr, canavanine and 5FOA resistant.