TABLE 1.

Mass spectrometric analysis of proteins copurifying with Smc5/6 complex factorsa

ProteinPurification of:
Smc5-TAPNse4-TAPNse6-TAP
No. of peptides% CoverageNo. of peptides% CoverageNo. of peptides% Coverage
Smc513281.6104555844.9
Smc611875.614468.76650.4
Nse11372.91039.4431.5
Nse23071.52863.71747.2
Nse31242.41936933.5
Nse45191533.7517.3
Nse5 (SPBC651.10)210.8310.11849.5
Nse6 (SPAC11E3.08c)316.52033.13861.5
  • a The indicated proteins were recovered in a single-step purification from fission yeast lysates on IgG-Sepharose columns. Eluted complexes were analyzed by mass spectrometry. The number of independent peptides of each protein recovered is indicated. The percentage of primary sequence covered by these peptides is also given.