TABLE 1.

Genotypes of progeny from heterozygous intercrossing

Stage (n)No. (%) with genotypea
+/++/−−/−
Newborn P28 (133)40 (30)93 (70)0 (0)
Embryo E10.5 (16)5 (34)11 (66)0 (0)
Blastocyst E3.5 (65)17 (26)48 (74)0 (0)
  • a Genotyping of each developmental stage was performed by PCR. A homozygous mutant was not observed. The frequency departs from the expected ratio of 1:2:1 for +/+ versus +/− versus −/− at each stage.