TABLE 3.

Analysis of chromosomal abnormalities in Tnks2PDΔ (−/−) and wild-type (+/+) ES cells

GenotypeCell lineapdNo. of metaphasesNo. of:
Signal free ends/ total chromosomesbEnd-to-end fusions/ total chromosomesChromosome breakages/ total chromosomes
Tnks2PDΔ (+/+)WT42500/1,9880/1,9880/1,988
Tnks2PDΔ (+/+)WT212500/1,9922/1,9922/1,992
Tnks2PDΔ (−/−)A342500/2,0560/2,0560/2,056
Tnks2PDΔ (−/−)A3212500/2,0831/2,0831/2,083
Tnks2PDΔ (−/−)C1042500/2,0431/2,0431/2,043
Tnks2PDΔ (−/−)C10212500/1,9853/1,9850/1,985
  • a A3 and C10 are independently derived Tnks2PDΔ (−/−) ES cells.

  • b For detection of signal free ends, the telomere images were overexposed to intensify weak telomeric fluorescent signals.