TABLE 1.

Library of histone alleles engineered through site-directed mutagenesis

HistoneResidueModificationSubstitutions
Histone H4Lys 31AcetylationK31A, K31R, K31Q
Ser 47PhosphorylationS47A, S47E
Lys 59MethylationK59A, K59R, K59Q
Lys 77AcetylationK77A, K77R, K77Q
Lys 79Acetylation/methylationK79A, K79R, K79Q
Lys 91AcetylationK91A, K91R, K91Q
Arg 92MethylationR92A, R92K
Histone H3Arg 52MethylationR52A, R52K, R52Q
Arg 53MethylationR53A, R53K, R53Q
Lys 56Acetylation/methylationK56A, K56R, K56Q
Lys 79MethylationK79A, K79R, K79Q
Lys 115AcetylationK115A, K115R, K115Q
Thr 118PhosphorylationT118A, T118E
Lys 122AcetylationK122A, K122R, K122Q