TABLE 1.

S. pombe anaerobically induced genes

Functional category and genedS. cerevisiae orthologaDescriptionbFold changec
WT −/+ O2WT/sre1Δ
Amino acid metabolism
    cys11+YGR012WCysteine synthase2.361.35
    SPAC26F1.07YPR1, GCY1Aldo/keto reductase2.071.35
    SPCC584.01cMET10Sulfite reductase NADPH flavoprotein subunit2.011.04
Carbohydrate metabolism
    gpd1+GPD1Glycerol-3-phosphate dehydrogenase2.320.88
    tdh1+TDH3, TDH1, TDH2Glyceraldehyde-3-phosphate dehydrogenase2.080.73
    SPACUNK4.16cTSL1, TPS3α,α-Trehalose-phosphate synthase2.050.81
    gpd3+TDH1, TDH3Glyceraldehyde-3-phosphate dehydrogenase2.030.87
    SPAC750.08cMAE1NAD-dependent malic enzyme2.020.81
    mae2+MAE1Malic enzyme2.000.82
Electron transport
    SPAC17A2.05OSM1, YEL047CFumarate reductase3.044.21
    SPBC23G7.10cNADH-dependent flavin oxidoreductase2.711.49
    SPCC970.03CBR1, YML087C, YML125CCytochrome b5 reductase2.241.93
    SPAC26H5.09cYMR315WOxidoreductase2.090.73
Ergosterol metabolism
    SPAC1687.16cERG3C-5 sterol desaturase3.922.36
    erg25+ERG25C-4 methylsterol oxidase3.143.05
    erg11+ERG11Cytochrome P450; C-14 lanosterol demethylase2.941.29
    erg5+ERG5Cytochrome P450; C-22 sterol desaturase2.761.34
    SPAC25B8.01DAP1MAPR family steroid-binding protein2.614.10
    erg1+ERG1Squalene monooxygenase2.470.93
    SPAC23H4.01cSWH1, OSH2, OSH3Oxysterol binding protein2.320.84
    SPCP1E11.05cARE1, ARE2Sterol O-acyltransferase2.221.65
    erg6+ERG6Δ-Sterol C-24 methyltransferase2.201.77
Fatty acid metabolism
    cut6+HFA1, ACC1Acetyl-CoA carboxylase2.180.83
    SPAC4H3.08FOX2Short-chain dehydrogenase2.171.16
Heme biosynthesis
    hem13+HEM13Coproporphyrinogen III oxidase14.0219.44
    hem15+HEM15Ferrochelatase5.4210.00
    hem14+HEM14Protoporphyrinogen oxidase2.093.09
Lipid metabolism
    SPBP4H10.11cFAA1, FAA2, FAA3Long-chain fatty acid-CoA ligase3.271.56
    SPAC589.09CSR1sec14 cytosolic factor family protein2.671.97
    SPAC8F11.08cEsterase/lipase2.101.74
    SPBC16A3.10YOR175CMembrane-bound O-acyltransferase2.091.71
    lcf1+FAA1, FAA3, FAA4Long-chain fatty acid-CoA ligase activity2.051.01
Sphingolipid metabolism
    SPBC887.15cSUR2Sphingosine hydroxylase5.514.22
    SPBC32F12.01cISC1Sphingomyelinase family protein2.962.17
    SPAC19G12.08SCS7Fatty acid hydroxylase2.143.00
Transcription
    sre1+Membrane-tethered transcription factor4.7517.30
Other
    dam1+DAM1DASH complex subunit5.042.86
    SPBC215.11cAldo/keto reductase4.492.53
    rga5+SAC7, BAG7GTPase activating protein4.234.18
    mmd1+LIA1Required for mitochondrial morphology and distribution3.765.56
    SPBC106.12cRNA-binding protein3.053.04
    SPAC10F6.01cECM17Sulfite reductase β-subunit3.010.97
    zym1+CRS5Zinc metallothionein2.542.78
    SPCC965.06Aldo/keto reductase2.541.22
    SPAC212.09cMalic enzyme pseudogene2.460.85
    SPBC6B1.08cYER049W2 OG-Fe(II) oxygenase superfamily protein2.431.59
    SPCC1259.02cYBR074WM28 peptidase family protein2.422.57
    SPAP8A3.02c2 OG-Fe(II) oxygenase superfamily protein2.181.96
    rds1+Protein involved in response to stress2.090.76
Unknown
    SPAC22A12.06cFSH2, FSH37.573.84
    SPBPB7E8.014.032.29
    SPAC18G6.01cFMP22, FMP343.775.64
    SPAC186.05c3.212.75
    SPBC21D10.06c2.851.18
    SPAC19B12.102.782.30
    SPAC1093.012.501.43
    SPAC2E1P3.05c2.413.01
    SPAC23C11.06cYNL115C2.361.48
    SPAC17A2.02cYJR116W, YPR114W2.321.28
    SPAC1565.01YNR018W2.062.09
    SPAC589.12CWH432.061.41
    SPCC1235.012.051.38
    SPAC18G6.12c2.032.63
    SPAC637.032.021.34
    SPAC13C5.06c2.012.75
  • a Predicted Saccharomyces cerevisiae ortholog (37).

  • b Descriptions were obtained from the S. pombe GeneDB (http://www.genedb.org/genedb/pombe/index.jsp) with some additional hand editing.

  • c Data are presented as the average changes in expression of genes in WT samples without O2 (WT − O2) compared to their expression in WT + O2 or sre1Δ − O2 samples.

  • d Statistically significant Sre1p-dependent genes are in boldface type (see Materials and Methods). Genes whose anaerobic induction was confirmed by Northern analysis are underlined.