TABLE 1.

Rescue of TFIIA function by the G269A mutant in X. laevis embryosa

ParametercMOαβMOαβMO + xIIAwtR (150 ng)αβMO + xIIAwtR (300 ng)αβMO + G269AR (150 ng)αβMO + G269AR (300 ng)
No. of injected embryos in expt 1113217202926
    % Abnormalb 010029.425.024.134.6
    % Normal100070.675.075.965.4
No. of injected embryos in expt 2314845323033
    % Abnormalb 010035.618.643.327.3
    % Normal100064.481.356.772.7
Total no. of injected embryos428062525959
    % Abnormalb 010033.921.233.930.5
    % Normal100066.178.866.169.5
  • a Phenotypes were scored at stage 37.

  • b The abnormal phenotypes observed in MO-injected embryos ranged from arrested gastrulation to severe axial defects.