TABLE 3.

Gene expression profiling analysis of progenitors expressing Vp16-Meis1 or coexpressing Hox>

Activation group and genebGene descriptionGenBank accession no.Transcript level scorea
a9 (n = 5)M+a9 (n = 5)VM (n = 3)VM+a9 (n = 3)VM+a7 (n = 3)
Weak to moderate activation by Vp16-Meis1, further activation by Hox
    Cd34Cd34 antigenNM_1336541373,2706104,8905,040
    Flt3Fms-like tyrosine kinase 3NM_010229<504,1429955,9401,992
    Il7rInterleukin-7 receptorNM_008372<506926801,0003,350
    CrlrCalcitonin receptor-like receptorAF209905<506901931,3051,785
    Msi2hMusashi homologue 2; RNA-binding proteinBB418489<507561453,8751,425
    Ergv-Est-related geneXM_622815<50884706251,775
    Sox4SRY box containing gene 4NM_0092385361,3761,0502,7951,310
    Gpr56G-protein-coupled receptor 56AK141886<4012650575990
    Tilz1bTSC22-related inducible leucine zipper 1bAF2012853701,9641,2506,3755,905
    PtprcapCD45-associated proteinNM_016933<501,1605201,4002,035
    Satb1Special AT-rich sequence-binding proteinNM_0091221541,5125821,5251,010
    Ly86MD1; lymphoid toll receptorNM_010745<501,3505131,9002,300
    Ngn/RC3Neurogranin, protein kinase C substrateAK0029332762,8961,6589,7156,185
    C1qR1Complement subcomponent 1q, receptor 1AK1503856303,0301,3084,9002,875
    Tmsb10Thymosin beta-10BB0963681,0806,3201,7186,3503,175
    Dbx4DEAD (Asp-Glu-Ala-Asp) box 4AK014844<501,968504,450115
    EdnraEndothelin receptor type AAK135555<50588502,3701,195
    P2rx3Purinergic receptor P2X, 3; ion channelBC023089<50496502,1501,000
    Kcna3Potassium voltage-gated channel 3AI32362484660210590400
    Wbscr15Transmembrane adaptor proteinAF2571361701,3742581,0751,500
    UnknownUnknownAV365503<504023954,875920
    UnknownUnknownBG071655<50418113550405
Activation by Vp16-Meis1, no superactivation by Hox
    Nrip1RIP140; Nuclear receptor-interacting proteinBB76455060310255283500
    Bcl2B-cell leukemia/lymphoma 2; antiapoptosisBI6644675142,6601,4151,4001,925
    Sesn3Sestin 3AK01746470836605825370
Activation by Hox only
    c-kitStem cell factor receptorX659972,3502,3908313,8502,675
Control
    GapdhGlyceraldehyde-3-phosphate dehydrogenaseBC08308012,89014,01611,02113,12314,125
    Actbβ-ActinAK14778713,53015,16813,20014,12516,450
  • a Score generated by the microarray scan program. M, Meis1; VM, Vp16-Meis1; a9, Hoxa9; a7, Hoxa7. The value of n represents the number of different immortalized progenitor lines (all cultured in SCF) that were used for analysis.

  • b Genes shown in bold have been implicated in the literature as either HSC markers or enriched in stem cell populations.