TABLE 2.

Selected genes whose expression is altered upon reduction of SnoN expressiona

Gene function and nameDescriptionRegulationb
Cell growth/proliferation
    CDKN1A (p21/Cip1)Cyclin-dependent kinase inhibitor 1AUp
    cyclin G2Regulation of cyclin-dependent protein kinase activityUp
    JunBjunB proto-oncogene, a negative regulator of cell proliferationUp
    IGFBP1Insulin-like growth factor binding protein 1Up
    GADD34Negative control of cell growthUp
    GADD45AGrowth arrest and DNA damage inducible, alphaUp
    cyclin E2Regulation of cyclin-dependent protein kinase activityDown
    cyclin A2Regulation of cyclin-dependent protein kinase activityDown
    cyclin D3Regulation of cyclin-dependent protein kinase activityDown
    E2FTranscription factor, regulation of cell cycleDown
    survivin-βNegative regulator of caspase 3, 7, and 9Down
    TIAF1TGFβ1-induced antiapoptotic factor 1Down
EMT/invasion/metastasis
    Twist1Twist homolog 1, regulator of EMTUp
    autotaxinAutocrine motility-stimulating factor (NPP-2), a metastasis-enhancing mitogenUp
    EGFREpidermal growth factor receptor, v-erb oncogene homologUp
    VEGFVascular endothelial growth factor, regulator of angiogenesisUp
    ORP150Oxygen-regulated protein, regulator of angiogenesisUp
    ST5Suppression of tumorigenicityUp
    SEL 1LHuman ortholog of Caenorhabditis elegans sel-1 geneUp
    TC10Small Ras-related GTPaseUp
    TGFBR3TGF-β receptor, type IIIDown
    GDI-1Rho GDP dissociation inhibitor, alphaDown
    CDKN2CCyclin-dependent kinase inhibitor 2C (p18), tumor suppressorDown
Adhesion/extracellular matrix
    FN1Fibronectin precursorUp
    ECM2Extracellular matrix protein 2Up
    integrin α2 β1Regulator of cell adhesion, migration, and invasionUp
    MMP16Matrix metalloproteinase 16, activator of MMP2Up
    DecorinExtracellular matrix component, substrate of MMP16Up
    PLAUPlasminogen activator, urokinaseUp
    Serpine 1/PAI-1Plasminogen activator inhibitor 1Up
    TJP2Tight junction protein 2Down
    EMP1Epithelial membrane protein 1Down
  • a mRNA was isolated from parental A549 and shSnoN-expressing cells and hybridized to Affymetrix GeneChips. Genes whose expression levels significantly increased or decreased in SnoN-deficient cells were sorted into functional groups.

  • b Genes whose signal was increased or decreased in A549 shSnoN cells compared with the levels in parental cells are annotated as “up” or “down,” respectively.