TABLE 1.

Strains and plasmids used in the present studya

Strain or plasmidaGenotype or relevant markersbSource or reference
Strains
    MS3212MATα leu2-3,112 his3Δ200 ura3-52 ade2-101 kar4Δ::HIS3
    MS3213MATa trp1Δ1 his3Δ200 ura3-52 ade2-101
    MS3215MATa leu2-3,112 his3Δ200 ura3-52 ade2-101 kar4Δ::HIS3
    MS3216MATa leu2-3,112 his3Δ200 ura3-52 ade2-101 kar4Δ::HIS3
    MY3265MATa ura3-52 trp1 his4-519 leu2 can1-101
    MY3375MATa ura3-52 leu2Δ1 his3Δ200
    MY4166MATa kar4Δ ura3-52 leu2Δ1 his3Δ200
    MY5100MATa ste12Δ::leu2Δ1 ura3-52 trp1 his4-519 leu2 can1-101
    MY5792MATa ura3-52 leu2Δ1 his3Δ200 KAR4::HA
    MY10133MATa ste12Δ::LEU2 trp1-901 leu2-3,112 ura3-52 his3Δ200 gal4Δ gal80Δ GAL2-ADE2 LYS2::GAL1-HIS3 met2::GAL7-lacZ
    PJ69-4AMATa trp1-901 leu2-3,112 ura3-52 his3Δ200 gal4Δ gal80Δ GAL2-ADE2 LYS2::GAL1-HIS3 met2::GAL7-lacZ19
    BY4741MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ049
    BY4742MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ049
    Y01430MATa prm2Δ::KanMX his3Δ1 leu2Δ0 met15Δ0 ura3Δ049
    Y11430MATα prm2Δ::KanMX his3Δ1 leu2Δ0 lys2Δ0 ura3Δ049
    Y03462MATa kar4Δ::KanMX his3Δ1 leu2Δ0 met15Δ0 ura3Δ049
    Y13462MATα kar4Δ::KanMX his3Δ1 leu2Δ0 lys2Δ0 ura3Δ049
Plasmids
    pLG669-ZlacZ 2μm URA315
    pMR2806PKAR3-L11-lacZ 2μm URA3
    pMR2802PKAR3-L16-lacZ 2μm URA3
    pMR2892PKAR3-L18-lacZ 2μm URA3
    pMR2808PKAR3-L14-lacZ 2μm URA3
    pMR2896PKAR3-L15-lacZ 2μm URA3
    pMR5620PKAR3-min-lacZ 2μm URA3
    pMR5621PKAR3-min31-2-lacZ 2μm URA3
    pMR5622PKAR3-min41-2-lacZ 2μm URA3
    pMR2654KAR4::HA CEN ARS URA3
    pMR2516KAR4 CEN ARS URA3
    pRS416CEN ARS URA345
    pMR5511kar4C214GC217G::HA CEN ARS URA3
    pMR3356PGAL-KAR4::HA-short CEN ARS URA3
    pMR3459PGAL-KAR4::HA-long CEN ARS URA3
    pGBD-C2GBD TRP1 2μm19
    pMR4997GBD-KAR4 TRP1 2μm
    pGAD-C2GAD LEU2 2μm19
    pMR5000GAD-KAR4 LEU2 2μm
    pMR5665Ptet-6xHN-KAR4; Cmr
    pMR5669Ptet-6xHN-STE12; Cmr
    pPROTet.E133Ptet-6xHN; CmrClontech
    pSUL16ste12Δ::LEU211
  • a Unless stated otherwise, all strains and plasmids were constructed in this laboratory.

  • b Cmr, chloramphenicol resistant.