DNA contents of mammary tumors occurring in three cohorts of Blm conditional knockout mice

Cohort and mouseGenotypeTumor typeDIaCVbDNA ploidycCell line stabilityd
    9907CKOFibroadenoma1.8, 3.48, 8AneuploidNAf
    1852CKOAdenocarcinoma1.4, 1.75, 8AneuploidNA
    7877CKOAdenocarcinoma1.3, 2.04, 8AneuploidNA
    7885CKOSquamous cell carcinoma1.14HyperdiploidNA
    7852CKODuctal carcinoma in situ1.34HyperdiploidNA
  • a DI, DNA index. Two values refer to two different stem lines.

  • b CV, coefficient of variation. Two values refer to two different stem lines.

  • c Tumors were defined as diploid if control organ and tumor G0/G1 DNA content peaks determined by flow cytometry were ≤10% of each other, hyperdiploid if there was a positive shift of 10 to 20%, and aneuploid if there was a shift of ≥ 30%. The minimum detectable DI as a function of the CV was established for each sample-control pair as described by Rabinovitch (42).

  • d From Results and Fig. 3.

  • e The mouse had multiple tumors, all of which were a mixture of adenocarcinoma, squamous cell carcinoma, and adenomyoepithelioma.

  • f NA, not applicable.

  • g Control bearing one wild-type allele and one tm4Ches floxed allele as well as the transgene.