TABLE 1.

DNA contents of mammary tumors occurring in three cohorts of Blm conditional knockout mice

Cohort and mouseGenotypeTumor typeDIaCVbDNA ploidycCell line stabilityd
Hs-cre
    1578CKOAdenomyoepithelioma1.113DiploidUnstable
    1579CKOAdenomyoepithelioma1.010DiploidUnstable
    1780CKOAdenomyoepithelioma0.910DiploidModerate
    1795CKOAdenomyoepithelioma0.912DiploidStable
    1776CKOAdenomyoepithelioma1.010DiploidStable
    1690CKOAdenomyoepithelioma0.98DiploidStable
    9907CKOFibroadenoma1.8, 3.48, 8AneuploidNAf
PSA-cre
    9261CKOAdenomyoepithelioma0.98DiploidUnstable
    9354CKOMixturee1.68AneuploidModerate
BLG-cre
    1852CKOAdenocarcinoma1.4, 1.75, 8AneuploidNA
    7877CKOAdenocarcinoma1.3, 2.04, 8AneuploidNA
    1983CKOAdenocarcinoma1.25HyperdiploidNA
    7725CKOAdenocarcinoma1.25HyperdiploidNA
    7885CKOSquamous cell carcinoma1.14HyperdiploidNA
    7852CKODuctal carcinoma in situ1.34HyperdiploidNA
    7724CKOAdenocarcinoma1.06DiploidNA
    1980ReportergAdenocarcinoma2.54AneuploidNA
  • a DI, DNA index. Two values refer to two different stem lines.

  • b CV, coefficient of variation. Two values refer to two different stem lines.

  • c Tumors were defined as diploid if control organ and tumor G0/G1 DNA content peaks determined by flow cytometry were ≤10% of each other, hyperdiploid if there was a positive shift of 10 to 20%, and aneuploid if there was a shift of ≥ 30%. The minimum detectable DI as a function of the CV was established for each sample-control pair as described by Rabinovitch (42).

  • d From Results and Fig. 3.

  • e The mouse had multiple tumors, all of which were a mixture of adenocarcinoma, squamous cell carcinoma, and adenomyoepithelioma.

  • f NA, not applicable.

  • g Control bearing one wild-type allele and one tm4Ches floxed allele as well as the transgene.