TABLE 2.

Germ line desilencing of a repetitive transgene in the his-24(ok1024) mutanta

CrossGeneration (his-24 genotype)% of hermaphrodites with desilencing effectb
PD7271 × his-24(ok1024) (male)F1 (ok1024/+)100 (87/87)
F2 (ok1024/ok1024)100 (10/10)
F3 (ok1024/ok1024)80 (177/142)
F4 (ok1024/ok1024)20 (100/20)
F5 (ok1024/ok1024)0 (100/0)
PD7271 (male) × his-24(ok1024)F1 (ok1024/+)100 (121/121)
F2 (ok1024/ok1024)100 (10/10)
F3 (ok1024/ok1024)70 (141/99)
F4 (ok1024/ok1024)10 (100/10)
F5 (ok1024/ok1024)0 (100/0)
Controls
    PD7271 × N2 (male)F1 (+/+)0 (531/0)
F2 (+/+)0 (101/0)
F3 (+/+)0 (378/0)
    PD7271 (male) × N2F1 (+/+)0 (341/0)
F2 (+/+)0 (110/0)
F3 (+/+)0 (211/0)
  • a The repetitive extrachromosomal transgene let-858::gfp was crossed from a his-24(+) source strain (PD7271) to the his-24(ok1024) mutant. Germ line transgene expression (Fig. 6) in ok1024 heterozygous F1 animals and in subsequent generations of his-24(ok1024) homozygous animals was analyzed.

  • b The numbers in parentheses are the number of animals analyzed followed by the number of animals with LET-858::GFP fluorescence in the germ line.