Table 5.

Growth of replication mutants in rad26Δ and cds1Δ backgrounds

AlleleTemp (°C)Mutator?aMutation typebViability in backgroundc
rad26Δcds1Δ
Wild-type30No++++++
polαts-11 25YesDelSLSL
polαts-13 25YesDelSL
polδts1 25YesDelSL+/−
cdc1-7 25YesDelSL+/−
cdc27-K3 30YesDel+/−
cdc17-K42 30YesDel
polδts3 30YesNDSCSL
cdc6-121 30YesNDSCSL+/−
rad2Δ30YesDup+++
polδts2 25No+++
cdc6-23 25NoSL+++
cdc20-M10 33NoND+++
cdc19-P1 30NoND+++
cdc23-M36 25NoND+++
cdc30 30NoND+++
  • a Strains that had a mutation rate at least fivefold greater relative to that of wild-type cells were designated as having a mutator phenotype.

  • b Del, deletion; Dup, duplication; NDSC, no detectable size change.

  • c Viability was measured by spotting 5-μl amounts of serially diluted cells onto YES (rich medium) plates incubated at various temperatures (see Materials and Methods). SL, synthetic lethal at 25°C; +++, viability indistinguishable from that of either of the parental strains; +/−, ∼10- to 100-fold-lower viability of the double mutant relative to that of either of the parental strains; −, ∼100- to 1,000-fold-lower viability of the double mutant relative to that of either of the parental strains. ND, not determined.