TABLE 1.

Strains used in this study

StrainGenotype (micronuclei)Phenotype (macronuclei)
CU428CHX1/CHX1 mpr1-1/mpr1-1 HTAX/HTAX HTA1/HTA1WT; Cys Mps Pms; VII
CU427chx1-1/chx1-1 MPR1/MPR1 HTAX/HTAX HTA1/HTA1WT; Cys Mps Pms; VI
B2086CHX1/CHX1 MPR1/MPR1 HTAX/HTAX HTA1/HTA1WT; Cys Mps Pms; II
HTAX S1P+5RΔhtaxhtax Δhta1hta1a CHX1/CHX1 mpr1?/mpr1?bH2A.X PRRRRR, ΔH2A.1; Pmr Cys Mp?cd
HTAX S1P+5R+(AAAAS)CΔhtaxhtax Δhta1hta1a CHX1/CHX1 mpr1?/mpr1?H2A.X PRRRRR+(AAAAS)C, ΔH2A.1; Pmr Cys Mp?d
HTAX S134A rescuedΔhtaxhtax Δhta1hta1a CHX1/CHX1 mpr1?/mpr1?H2A.X S134A, ΔH2A.1; Pmr Cys Mp?d
Rejuvenated HTAX S134A rescuedchx1-1/chx1-1 MPR1/MPR1 HTAX/HTAX HTA1/HTA1H2A.X S134A, ΔH2A.1; Pmr Cys Mp?d
HTAX rescuedΔhtaxhtax Δhta1hta1a CHX1/CHX1 mpr1?/mpr1?H2A.X, ΔH2A.1; Pmr Cys Mp?d
Somatic HTAX S134ACHX1/CHX1 mpr1-1/mpr1-1 HTAX/HTAX HTA1/HTA1 or CHX1/CHX1 MPR1/MPR1 HTAX/HTAX HTA1/HTA1H2A.X S134A, H2A.1; Pmr Cys Mps; VII or II
Somatic HTAXCHX1/CHX1 mpr1-1/mpr1-1 HTAX/HTAX HTA1/HTA1 or CHX1/CHX1 MPR1/MPR1 HTAX/HTAX HTA1/HTA1H2A.X, H2A.1; Pmr Cys Mps; VII or II
HTAX-neo3 somatic replacing S134A in S134A rescuedΔhtaxhtax Δhta1hta1a CHX1/CHX1 mpr1?/mpr1?H2A.X, ΔH2A.1; Pmr Cys Mp?d
  • a The full genetic nomenclature for Δhtaxhtax Δhta1hta1 is htax-1::neo2/htax-1::neo2 hta1-1::neo2/hta1-1::neo2 (2); the abbreviation has been used to conserve space.

  • b Question mark indicates undetermined genotype.

  • c Mp?, 6-methyl-purine sensitivity not determined.

  • d Mating type not determined.