Table 1.

Strains used in this study

Mutant proteinStrainTemp (°C)Refer-ence
PermissiveSemipermissiveNonpermissive
Wild-type 972 Not ts
FEN-1 rad2ΔNot ts 30
Polα polαts11 <2121–2530 3
Polα polαts13 <2121–2530 3
Polα pol1-1 <2121–2530 9
Polδ polδts1 212530 11
Polδ polδts2 212530 11
Polδ polδts3 253033 11
Polδ cdc6-23 212530 33
Polδ cdc6-121 253033 33
Polδ small subunit (p55) cdc1-7 212530 33
Polδ-associated subunit (p54) cdc27-K3 253033 32
Ligase cdc17-K42 253033 32
Polɛ cdc20-M10 303336 32
MCM2 cdc19-P1 253033 32
MCM10 cdc23-M36 212530 32
Orp1 cdc30 212530 13
Rad26 rad26ΔNot ts 1
Cds1a cds1ΔNot ts 29
RecQ rqh1ΔNot tested 35
  • a S. cerevisiae Rad53.