Table 1.

pacC mutations used in this work

MutationParental strain genotypeMutagenSelection methodReference
pacC504 pabaA1 pacC c 5 NQOa Growth on 50 mM GABAb as C and N sources 39
pacC+/−7604 inoB2 pacCc 76 UVResistance to neomycin (2 mg/ml) 39
pacC+/−508 and515 pabaA1 pacC c 5 UVResistance to neomycin (200 μg/ml) + 0.04% sodium deoxycholate 39
pacC+/−230 areA r inoB2 fwA1 UVGrowth on 5 mM GABA as N sourceThis work
pacC c 20000 yA2 gatA2 pantoB100 pacC c 200 UVResistance to neomycin (1 mg/ml)This work
pacC c 63 and69 pabaA1 yA2 wA3 palA1 UVGrowth at pH 8This work
pacC +/− 206 biA1 areA1601 areA r 168 pantoB100 UVGrowth on 5 mM GABA as N sourceThis work
pacC c/− 20601, 20602, and20604 pacC+/−206 pantoB100 UVGrowth at pH 8This work
pacC c 20603 pacC+/−206 pantoB100 UVGrowth at pH 8This work
pacC c 39 biA1 pyroA4 palB7 chaA1 NQOGrowth at pH 8 at 42°CThis work
pacC c 50 biA1 nis-5 UVEnhanced growth on nitrite as N source + 40 mM CsCl 29
pacC c 2020503 and -2020508(2020516, 2020515) pabaA1 pacC+/−20205 pacC c 202 UVGrowth at pH 8This work
pacC c 67 and 75 inoB2 palB7 chaA1 UVGrowth at pH 8This work
pacC c 202 pabaA1 sasA60 SpontaneousResistance to GABA toxicity 39
pacC+/−20205 pacC c 202 UVResistance to neomycin (1 mg/ml)This work
pacC c 14 pabaA1 gatA2 palF15 fwA1 UVResistance to 5 mM GABA in presence of 10 mM ammonium tartrate as N source 39
pacC c 5 biA1 UVDecreased staining for acid phosphatase 11
  • a NQO, 4-nitroquinoline-1-oxide.

  • b GABA, α-aminobutyrate.