Table 3.

Effect of multicopy SSA1 plasmid on [PSI]-mediated nonsense suppression in strain OT56

Plasmidβ-Galactosidase (ng)/mg of total proteinSuppression (%; mean ± SE)
Readthrough (pUKC815)UGA (pUKC819)
Control (YEp13)1,2934.350.34 ± 0.16
SSA1(pSSA1-LEU2)83512.51.41 ± 0.38
  • a Determined as ratio UGA/readthrough. At least four independent transformants were analyzed per each strain-plasmid combination; at least two measurements were performed per each transformant.