Table 1.

Activation of reporters by p53 and the p73 isoforms a

cis-acting elementp53 p73α p73β
None
RGC+++++
SCS+++++++++
p21+++++++++
mdm2+++++++++
cyclin G+++++++
GADD45+++++++
Bax+−/+−/+
IGF-BP3 box A
IGF-BP3 box B
  • a Strains expressing wild-type or mutant p53, p73α, and p73β (on a LEU2 plasmid) and containing (on a TRP1 plasmid) one of the p53-responsive reporters (RGC, SCS, p21, mdm2, cyclin G, GADD45, Bax, IGF-BP3 box A, IGF-BP3 box B) or control reporter (None) were (i) streaked out for single colonies onto SC-minus-leucine-minus-tryptophan-minus-histidine plates and grown for ∼2 days at 37°C and (ii) replica plated from SC-minus-leucine-minus-tryptophan plates grown at 30°C to SC-minus-leucine-minus-tryptophan-minus-histidine plates and grown for 1 to 2 days at 37°C. Growth of wild-type p73-containing strains on histidine-deficient medium was scored against growth of strains expressing wild-type p53 and the relevant reporter. TheLEU2/CEN (no p53, p73α, and p73β expression), p53R273H-, p73αR292H-, and p73βR292H-containing strains were inactive for transactivation with all reporters, scored −, −, no growth; −/+, slow growth; +, growth; ++, moderate growth; +++, wild-type growth.