TABLE 2.

Total hypoxia-induced cellular changes in gene expression

FunctionTranscriptional fold changeGene name and descriptionReference(s)
Perk+/+ cellsPerk−/− cells
Angiogenesis7.95.8VEGF A3, 29, 43
4.93.1Adrenomedullin 218, 45, 53
4.74.4Heme oxygenase 170
3.62.2Angiopoietin-like 6
3.63.3Ephrin B247
1.82.4Angiomotin
NO mediated22.13.3Metallothionein 18, 69
18.72.4Metallothionein 28, 69
UPR13.815.4hsp1A
10.126.6hsp1A
4.56.3hsp1
3.54.4hsp70
6.32.2Myd116 (Gadd34)9
1.92.5DnaJ (Hsp40) homolog
11.56.0Ddit3 (CHOP)7, 9, 55
Regulation of transcription15.618.6ATF32, 9, 76
3.02.0ATF5
4.82.5Max dimerization protein
3.41.8Max protein
Metabolism8.62.6CA6
6.31.9Endothelial cell growth factor 1
3.25.2Adenylate kinase 43, 95
2.21.9Aldehyde dehydrogenase 272
2.02.06-Phosphofructo-2-kinase/fructose-2, 6-Biphosphatase 3
2.02.2Phosphoglucomutase 2
3.44.0Hexokinase 281, 64
2.22.7Pyruvate carboxylase101
3.01.8Asparagine synthetase
Response to DNA damage10.95.6DNA damage-inducible transcript 3
6.94.8GADD45a85
5.18.3DNA damage-inducible transcript 4
Apoptosis4.82.5Max dimerization protein
3.418.6NIP312, 30, 89
2.52.8BCL2-like 11 (apoptosis facilitator)
mRNA processing and translation2.22.5Cytoplasmic polyadenylation element Binding protein 4
2.11.9Dual-specificity phosphatase 3
  • Results of Affymetrix MG430_2 microarray analysis used to identify steady-state changes in gene expression during hypoxia. Genespring 5.0 software was used to compare Perk+/+ and Perk−/− 4 h total to 0 h total RNA to determine gene changes induced >2-fold. Genes were functionally clustered and referenced if previously identified in the literature. Only named genes are included in this list; expressed sequence tags were omitted.