TABLE 1.

Data concerning the top 23 stabilized transcripts in the TTP-KO cellsa

EST clone used in Northern blots (GenBank accession no.)mRNA size (kb)bStabilization in TTP-KO cells (66−/−)cRankAccession no. (mRNA size in bp)dName
BI1904834∼1.2++++1NM_133662 (1,110)Immediate-early response 3 (Ier3)
BI1687808∼2.52NM_009895 (2,161)Cytokine-inducible SH2-containing protein (Cish)
BM949069∼1.5+++3NM_019914 (2,018)Myeloid/lymphoid or mixed-lineage leukemia (trithorax) (Mllt11)
BC064016Undetectable4NM_010096 (2,880)Early B-cell factor 3 (Ebf3)
BQ959150∼4++ (90 min), ++ (45 min)5NM_008501 (5,850)Leukemia inhibitory factor (Lif)
BM234765∼2.8+6NM_152804 (2,804)Polo-like kinase 2 (Plk2)
BM213337∼1.2++ (90 min), +++ (45 min)7XM_127501 (6,152)B double-prime 1 (Bdp1),
BU515336∼28NM_020579 (1,952)UDP-Gal:βGlcNAc β1,4-galactosyltransferase (B4galt3)
BQ573408∼2.5+9NM_145478 (2,447)Proviral integration site 3 (Pim3)
BM240684∼6 and ∼1.2− (6 kb) and + (1.2 kb)10AK033720 (2,364)Membrane protein CH1
BQ178289∼511NM_153078 (4,820)EH domain binding protein 1 (Ehbp1)
BU523505∼2.8+++12NM_013807 (2,648)Polo-like kinase 3 (Plk3)
BI729838∼6+13XM_131380 (5,279)RUN- and SH3 domain-containing 2 (Rusc2)
BQ939486∼1.9+/−14NM_009344 (1,954)Pleckstrin homology-like domain, family A, member 1 (Phlda1)
BE198619∼115NM_008176 (884)Chemokine (C-X-C motif) ligand 1 (Cxcl1)
BM250978∼616NM_182995 (5,024)RIKEN cDNA 6330503K22 gene
BM219304∼2.5?17AK079543 (2,333)Regulator of G protein 16
BE335087∼618NM_172303 (5,385)PHD finger protein 17 (Phf17)
CA3177693 and 719XM_129477 (5,385)Calmodulin-regulated spectrin-associated protein 1-like 1, transcript variant 1 (Camsap1l1)
AI0372213.820NM_172845 (3,673)Disintegrin-like and metallopeptidase (reprolysin; Adamts4)
AI604004∼421XM_485240 (4,140)TSC22 domain family 2 (Tsc22d2)
BU555856∼4.522NM_007930 (4,695)Ectodermal-neural cortex 1 (Enc1)
BQ9648192.523NM_009423 (4,107)Tnf receptor-associated factor 4 (Traf4)
  • a The transcripts are listed in order of decreasing SAM scores; these scores, and the decay curves developed from the microarray experiment, are shown in Fig. S2 in the supplemental material. The number of + symbols in the third column of this table refers to the extent of stabilization of the transcript in the absence of TTP, as determined by Northern blotting experiments, with ++++ representing maximal stabilization.

  • b As determined by Northern blotting in this work.

  • c As determined by Northern blotting following serum stimulation and actinomycin D addition. All Northern blots were analyzed by PhosphorImager and corrected according to α-tubulin or GAPDH hybridized to the same blot.

  • d As suggested by the GenBank accession number.