Table 2.

Expression of promoter-reporter constructs is reduced in the paf1Δ straina

Strainβ-Galactosidase activity (U/mg) with promoter:
CYC1FKS1
Wild type24,000 ± 1,50020,000 ± 3,200
paf1Δ1,900 ± 3702,600 ± 150
  • a Extracts from wild-type (YJJ662) andpaf1Δ mutant (YJJ664) strains bearing the pF712-380pFKS1-lacZ reporter plasmid (25) or the AJ-1CYC1-lacZ reporter plasmid (28) were prepared and assayed as described in Materials and Methods. β-Galactosidase activity (± the standard error of the mean) is expressed in units per milligram of protein, where 1 U is defined as the A420 × 103 per minute. Values are derived from replicate assays of at least three different transformants.