TABLE 1.

S. cerevisiae strains used in this study

StrainGenotypeaSource or reference
JDY4his3Δ200 leu2Δ1 trp1Δ99 ade2-101 ura3Δ99 ciro MATα30
MTY557/2aleu2-3,112 ade2-1 ura3-1 MATaI. Stansfield
MTY557/1dleu2-3,112 ade2-1 ura3-1 sup45-2 MATaI. Stansfield
BSC483/1ahis5-2 lys1-1 ade2-1 ura3-1 can1-100 SUQ5 PNM1 MATαI. Stansfield
BSC483/1a-sal4-42his5-2 lys1-1 ade2-1 ura3-1 can1-100 SUQ5 PNM1 sup45-42 MATαI. Stansfield
YDB498bhis3-11,15 leu2-3,112 trp1-1 ade1-14 ura3-1 sup35::HIS3 pPW12.1 MATa42
74-D694his3Δ200 leu2-3,112 trp1-289 ade1-14 ura3-52 MATaM. Tuite
74-D694[PSI+]schis3Δ200 leu2-3,112 trp1-289 ade1-14 ura3-52 MATa [PSI+]strongM. Tuite
74-D694[PSI+]wchis3Δ200 leu2-3,112 trp1-289 ade1-14 ura3-52 MATa [PSI+]weakM. Tuite
CSY550leu2Δ1 trp1Δ63 ura3-52 rat8-2C. Cole
HFY870his3-11,15 leu2-3,112 trp1-1 ade2-1 ura3-1 can1-100 upf1::HIS3A. Jacobson
CML476his3Δ200 leu2Δ1 ura3-52 CMVp(tetR′-SSN6)::LEU2 trp1::tTAN. Zhang
JDY808his3Δ200 leu2Δ1 ura3-52 CMVp(tetR′-SSN6)::LEU2 trp1::tTA sup45::kanMX4-tetO7-SUP45This work
  • a All strains are [psi], unless otherwise noted.

  • b Strains expressing eRF3 variants were generated by transforming YDB498 with plasmids encoding Sup35p or the H348Q or R419G mutant protein and evicting pPW12.1 on 5-fluoroorotic acid plates.

  • c Weak and strong [PSI+] 74-D694 variants were characterized in the laboratory of M. Tuite (Kent, United Kingdom).