TABLE 1.

FGF regulation of Wnt target genes in osteoblasts

Wnt target gene characteristicaGene symbolAccession no.Fold induction seen for treatment withb:Description
Wnt3aWnt3a + FGF1FGF1
FGF inhibited; not significantly A930038C07Rik BE63285028.732.331.50Fibronectin type III-like fold
    regulated by FGF alone Has2 NM_008216 11.773.530.63Hyaluronan synthase 2
Rhou AF378088 8.761.810.86 ras homolog gene family, member U
Tcf7 (Tcf1) AI323642 5.741.640.66Transcription factor 7; T cell specific
Sstr2 NM_009217 5.381.691.37Somatostatin receptor 2
Fndc1 AK003938 4.661.130.79Fibronectin type III domain containing 1
Ahr BE989096 4.541.681.35Aryl-hydrocarbon receptor
Ctgf NM_010217 3.771.160.63Connective tissue growth factor
Mab21l1 AF228913 3.760.560.50 mab-21-like 1
Gdnf NM_010275 3.580.700.80Glial cell line-derived neurotrophic factor
1500005K14Rik BF1017213.440.780.44RIKEN cDNA 1500005K14 gene
Slc7a2 AV244175 3.351.130.48Solute carrier family 7, member 2
Ifi1 NM_008326 3.160.670.55Interferon-inducible protein 1
Tmem23 BE629162 2.841.120.69Transmembrane protein 23
Cyr61 BB533736 2.740.720.48Cysteine-rich protein 61
Zfpn1a2 BB291816 2.690.890.47Zinc finger protein, subfamily 1A, 2 (Helios)
FGF inhibited; downregulated Edn1 D43775 16.830.170.19Endothelin 1
    by FGF alone Axin2 BB398993 10.223.160.19Axin2
Tgfb3 BC014690 9.920.530.23Transforming growth factor, beta 3
Timp3 BI111620 6.390.470.15Tissue inhibitor of metalloproteinase 3
EST BI076661 5.150.480.07Clone 9030002L20
Gadd45g AK007410 4.800.440.20Growth arrest- and DNA-damage-inducible 45 gamma
Serpinb1a AF426024 4.710.040.02Serine proteinase inhibitor, clade B, member 1a
Tmepai AV291712 4.011.390.30Transmembrane, prostate androgen-induced RNA
Thbs1 AI385532 3.590.340.17Thrombospondin 1
2310039E09Rik NM_026509 3.390.110.10RIKEN cDNA 2310039E09 gene
Irs1 BB345784 3.360.810.37Insulin receptor substrate 1
EST BM229155 3.190.900.26Transcribed sequences
FHOS2 BG066491 2.990.410.33Formin-family protein FHOS2
Prickle1 BC022643 2.870.960.33Prickle-like 1
Lrrc17 BB503935 2.860.350.17Leucine-rich repeat containing 17
Acpl2 BB183525 2.850.870.29Acid phosphatase-like 2
Tgfb2 BF144658 2.800.170.07Transforming growth factor B2
4930579P08Rik AK016333 2.580.140.14Clone 4930579P08
Variably affected by FGF-Wnt Dusp4 AK012530 7.5214.116.75Dual-specificity phosphatase 4
    combination Sema4f BB271145 6.753.290.41Semaphorin 4f
2610035D17Rik BB760848 4.842.181.07RIKEN cDNA 2610035D17 gene
Il33 NM_133775 4.368.885.69Interleukin 33
Irx3 NM_008393 4.113.390.98Iroquois-related homeobox 3
EST BB042558 3.642.262.24EST
Arl4c BI964400 3.632.971.77ADP ribosylation factor-like 4c
Grem1 BC015293 3.623.223.05Cysteine knot superfamily 1, BMP antagonist 1
Flvcr1 BC010797 3.493.631.78Feline leukemia virus subgroup C cellular receptor 1
Ier3 NM_133662 3.379.107.28Immediate early response 3
Nudt6 BB043522 3.2612.564.41Nudix (nucleoside diphosphate linked moiety X)-type motif 6
Sema3c NM_013657 3.255.342.63Semaphorin 3C
Cxcl1 NM_008176 3.191.311.17Chemokine (C-X-C motif) ligand 1
Dyrk3 BC006704 3.193.793.10Dual-specificity tyrosine-(Y) phosphorylation-regulated kinase 3
Prdc NM_011825 3.043.873.40Protein related to DAN and cerberus
Fmnl2 AK017338 2.992.621.85Formin-like 2
4931408A02Rik AK016443 2.941.951.16RIKEN cDNA 4931408A02 gene
Tmem16a AU040576 2.9311.146.06Transmembrane protein 16a
Ptar1 AK008237 2.912.102.09Protein prenyltransferase alpha subunit repeat containing 1
Gas2l3 BB770972 2.791.461.45Growth arrest-specific 2-like 3
Tcf7l2 (Tcf4) BB175494 2.781.190.84Transcription factor 7-like 2; T cell specific; HMG-box
Sema5a AV375653 2.764.574.29Semaphorin 5a
EST AV352204 2.731.230.86Transcribed sequences
Inhba NM_008380 2.671.671.28Inhibin beta-A
Runx1 D13802 2.643.873.54Runt-related transcription factor 1
Ptgs2 M94967 2.625.603.60Prostaglandin-endoperoxide synthase 2
EST BB433596 2.621.700.83Transcribed sequences
Sox4 BG083485 2.602.000.82SRY-box containing gene 4
Zbtb38 BE456566 2.592.071.23Zinc finger and BTB domain containing 38
Tnfrsf21 BG972377 2.581.571.25Tumor necrosis factor receptor superfamily, member 21
C79267 BG066466 2.574.063.53Expressed sequence C79267
Adam12 NM_007400 2.561.630.83Disintegrin and metalloproteinase domain 12
2010002N04Rik BI963682 2.556.133.98RIKEN cDNA 2010002N04 gene
  • a Genes induced by Wnt3a are listed in order of decreasing change. Those genes significantly downregulated in combination with FGF1 are grouped according to the effect of FGF1 alone on their expression levels. The third group consists of those genes variably affected in combination with FGF1. Note that several of these genes are downregulated by FGF but by less than 2.5 fold.

  • b Changes in gene expression in OB-TOP#1 cells treated with Wnt3a, Wnt3a plus FGF1, and FGF1 at 6 h relative to levels in untreated cells.