TABLE 1.

FGF regulation of Wnt target genes in osteoblasts

Wnt target gene characteristicaGene symbolAccession no.Fold induction seen for treatment withb:Description
Wnt3aWnt3a + FGF1FGF1
FGF inhibited; not significantlyA930038C07RikBE63285028.732.331.50Fibronectin type III-like fold
    regulated by FGF aloneHas2NM_00821611.773.530.63Hyaluronan synthase 2
RhouAF3780888.761.810.86ras homolog gene family, member U
Tcf7 (Tcf1)AI3236425.741.640.66Transcription factor 7; T cell specific
Sstr2NM_0092175.381.691.37Somatostatin receptor 2
Fndc1AK0039384.661.130.79Fibronectin type III domain containing 1
AhrBE9890964.541.681.35Aryl-hydrocarbon receptor
CtgfNM_0102173.771.160.63Connective tissue growth factor
Mab21l1AF2289133.760.560.50mab-21-like 1
GdnfNM_0102753.580.700.80Glial cell line-derived neurotrophic factor
1500005K14RikBF1017213.440.780.44RIKEN cDNA 1500005K14 gene
Slc7a2AV2441753.351.130.48Solute carrier family 7, member 2
Ifi1NM_0083263.160.670.55Interferon-inducible protein 1
Tmem23BE6291622.841.120.69Transmembrane protein 23
Cyr61BB5337362.740.720.48Cysteine-rich protein 61
Zfpn1a2BB2918162.690.890.47Zinc finger protein, subfamily 1A, 2 (Helios)
FGF inhibited; downregulatedEdn1D4377516.830.170.19Endothelin 1
    by FGF aloneAxin2BB39899310.223.160.19Axin2
Tgfb3BC0146909.920.530.23Transforming growth factor, beta 3
Timp3BI1116206.390.470.15Tissue inhibitor of metalloproteinase 3
ESTBI0766615.150.480.07Clone 9030002L20
Gadd45gAK0074104.800.440.20Growth arrest- and DNA-damage-inducible 45 gamma
Serpinb1aAF4260244.710.040.02Serine proteinase inhibitor, clade B, member 1a
TmepaiAV2917124.011.390.30Transmembrane, prostate androgen-induced RNA
Thbs1AI3855323.590.340.17Thrombospondin 1
2310039E09RikNM_0265093.390.110.10RIKEN cDNA 2310039E09 gene
Irs1BB3457843.360.810.37Insulin receptor substrate 1
ESTBM2291553.190.900.26Transcribed sequences
FHOS2BG0664912.990.410.33Formin-family protein FHOS2
Prickle1BC0226432.870.960.33Prickle-like 1
Lrrc17BB5039352.860.350.17Leucine-rich repeat containing 17
Acpl2BB1835252.850.870.29Acid phosphatase-like 2
Tgfb2BF1446582.800.170.07Transforming growth factor B2
4930579P08RikAK0163332.580.140.14Clone 4930579P08
Variably affected by FGF-WntDusp4AK0125307.5214.116.75Dual-specificity phosphatase 4
    combinationSema4fBB2711456.753.290.41Semaphorin 4f
2610035D17RikBB7608484.842.181.07RIKEN cDNA 2610035D17 gene
Il33NM_1337754.368.885.69Interleukin 33
Irx3NM_0083934.113.390.98Iroquois-related homeobox 3
ESTBB0425583.642.262.24EST
Arl4cBI9644003.632.971.77ADP ribosylation factor-like 4c
Grem1BC0152933.623.223.05Cysteine knot superfamily 1, BMP antagonist 1
Flvcr1BC0107973.493.631.78Feline leukemia virus subgroup C cellular receptor 1
Ier3NM_1336623.379.107.28Immediate early response 3
Nudt6BB0435223.2612.564.41Nudix (nucleoside diphosphate linked moiety X)-type motif 6
Sema3cNM_0136573.255.342.63Semaphorin 3C
Cxcl1NM_0081763.191.311.17Chemokine (C-X-C motif) ligand 1
Dyrk3BC0067043.193.793.10Dual-specificity tyrosine-(Y) phosphorylation-regulated kinase 3
PrdcNM_0118253.043.873.40Protein related to DAN and cerberus
Fmnl2AK0173382.992.621.85Formin-like 2
4931408A02RikAK0164432.941.951.16RIKEN cDNA 4931408A02 gene
Tmem16aAU0405762.9311.146.06Transmembrane protein 16a
Ptar1AK0082372.912.102.09Protein prenyltransferase alpha subunit repeat containing 1
Gas2l3BB7709722.791.461.45Growth arrest-specific 2-like 3
Tcf7l2 (Tcf4)BB1754942.781.190.84Transcription factor 7-like 2; T cell specific; HMG-box
Sema5aAV3756532.764.574.29Semaphorin 5a
ESTAV3522042.731.230.86Transcribed sequences
InhbaNM_0083802.671.671.28Inhibin beta-A
Runx1D138022.643.873.54Runt-related transcription factor 1
Ptgs2M949672.625.603.60Prostaglandin-endoperoxide synthase 2
ESTBB4335962.621.700.83Transcribed sequences
Sox4BG0834852.602.000.82SRY-box containing gene 4
Zbtb38BE4565662.592.071.23Zinc finger and BTB domain containing 38
Tnfrsf21BG9723772.581.571.25Tumor necrosis factor receptor superfamily, member 21
C79267BG0664662.574.063.53Expressed sequence C79267
Adam12NM_0074002.561.630.83Disintegrin and metalloproteinase domain 12
2010002N04RikBI9636822.556.133.98RIKEN cDNA 2010002N04 gene
  • a Genes induced by Wnt3a are listed in order of decreasing change. Those genes significantly downregulated in combination with FGF1 are grouped according to the effect of FGF1 alone on their expression levels. The third group consists of those genes variably affected in combination with FGF1. Note that several of these genes are downregulated by FGF but by less than 2.5 fold.

  • b Changes in gene expression in OB-TOP#1 cells treated with Wnt3a, Wnt3a plus FGF1, and FGF1 at 6 h relative to levels in untreated cells.