Table 4.

Mutation rates and types of mutations of ts replication mutators in rad2 + andrad2Δ backgroundsa

AlleleTemp (°C)Relative mutation rateb, c% Mutation type in cellsc, d
DelDupNDSC
rad2+ rad2Δrad2+ rad2Δrad2+ rad2Δrad2+ rad2Δ
Wild-type301.0 16.5 0503710058
polαts11 25185.9 40.6 85 6 0 6 15 88
polαts13 25169.0 23.7 61 5 6 0 33 95
polδts1 2511.5 6.7 94 0 0 14 6 86
polδts2 25≤1.0 20.3 ND
polδts3 3022.3 19.0 ND
cdc1-7 259.1 12.7 26 0 0 0 74 100
cdc27-K3 3010.1 13.2 24 6 0 6 76 88
cdc17-K42 3013.5 6.1 48 7 0 33 52 60
  • a All mutation assays were performed at the semipermissive temperature (see Table 1) for each strain or at 30°C for the wild-type strain.

  • b The relative mutation rate is the rate compared to that of wild-type cells.

  • c Mutation rates and percentages of mutations in a rad2Δ background are shown in boldface. The remaining entries are from Table 2.

  • d Del, deletion; Dup, duplication; NDSC, no detectable size change; ND, not determined.