Table 1.

Kinetic analysis of recombinant IKK proteinsa

Proteinkcat(h−1)Km (μM) for:
IκBα 1–54IκBα 1–317IκBβ 1–44ATP
IKK23.30.5NDb 1.10.56
IKK2EE33.81.10.13.00.63
IKK10.781.4ND2.20.63
  • a Kinase reactions were performed with purified baculovirus-expressed IKK proteins in a 96-well microplate format (for 1 h at room temperature in a 0.1-ml volume). In all cases, GST IκBα 1–54 WT was used as the substrate.

  • b ND, not done.