TABLE 1.

Sorting by MPR300 and MPR/SorLA chimeras in mpr−/− cellsa

ConstructLevel of expressionc ± SDMean % extracellular β-hexosaminidaseb of total ± SD:
Without M6PWith M6P
Nontransfected85.04 ± 2.4285.31 ± 0.46
MPR3002.23 ± 0.0911.43 ± 1.7324.79 ± 0.44
MPR300/SorLA constructs
    wt-tail1.93 ± 0.0814.33 ± 1.0822.06 ± 1.83
    Δ[30-54]2.76 ± 0.1645.23 ± 1.9653.68 ± 1.66*
    D30D,E34DDED to A's1.51 ± 0.1031.68 ± 1.9257.99 ± 1.53*
    M41I to A's2.46 ± 0.1319.93 ± 2.0845.82 ± 3.10*
    D47 to A2.85 ± 0.0911.02 ± 1.1532.09 ± 3.07*
    D48 to A2.44 ± 0.1018.00 ± 0.4537.55 ± 0.74*
    M51 to A1.75 ± 0.1415.33 ± 2.1943.94 ± 1.21*
  • a All values represent means of three separate experiments performed in quadriplicates.

  • b The percent activity in culture medium without (−) or with (+) 5 mM M6P is given. *, P < 0.01 on comparison with wt-tail (a one-way analysis of variance and a post hoc Bonferroni t test was used).

  • c When endogenous MPR300 expression in MEF cells is set to 1.