TABLE 3.

Suppression of the maf1-Δ phenotype by various Pol III mutationsa

Pol III mutantDefect in Pol III transcriptionMutated Pol III subunitSuppression of maf1Fold decrease in tRNA levels
rpc31-236InitiationC31+2.5
rpc25-S100PInitiationC252.6
rpc160-112ElongationC1601.2
rpc160-750TerminationC160+1.4
rpc11-SpTerminationC11+1.3
rpc128-1007Not studiedC128+1.6
  • a To determine suppression of the maf1-Δ phenotype, Pol III mutants with a deleted chromosomal MAF1 gene and respective control strains were grown on YPD medium overnight, replica plated on YPGly medium, and incubated for 3 days at 37°C. To estimate the decrease in tRNA levels, total RNA was separated by electrophoresis. Intensities of tRNA bands stained with ethidium bromide were quantified using Image Quant 6.2 software.