TABLE 1.

Wild-type and mutant sequence probes representing the seven E box sites present in the first PHM introna

E box(es) and probeSequence
E1
    E1 box5′-cgc tcCATATGga tga-3′
    E1M box5′-cgc tcTATACTga tga-3′
    E1 to 2 box5′-cgc ccCAGCTGca tga-3′
    E1 to 7 box5′-cgc ccCATTTGaa tga-3′
E2 and E3
    E23 box5′-tcg ccCAGCTG CAGCTGtg cgc-3′
    E23M box5′-tcg ccTAGCCT TAGCCTtg cgc-3′
    E2M box5′-tcg ccTAGCCT CAGCTGtg cgc-3′
    E3M box5′-tcg ccCAGCTG TAGCCTtg cgc-3′
    E23 to 7 box5′-tcg ccCATTTG CATTTGaa cgc-3′
E4 and E5
    E45 box5′-ttt tcCATATGt tCAGTTGta ccc-3′
    E45M box5′-ttt tcTATACTt tTAGTCTta ccc-3′
    E4M box5′-ttt tcTATACTt tCAGTTGta ccc-3′
    E5M box5′-ttt tcCATATGt tTAGTCTta ccc-3′
    E45 to 7 box5′-tcg ccCATTTGt tCATTTGaa cgc-3′
E6
    E6 box5′-cca ccCACATGta agg-3′
    E6M box5′-cca ccTACACTta agg-3′
E7
    E7 box5′-act ccCATTTGaa taa-3′
    E7M box5′-act ccTATTCTaa taa-3′
  • a The 6-bp E-box sequences are in uppercase and in order as named in column 1. Sequence alterations are shown in bold. Underlines indicate the final extent of sequence matching to the target E-box site (e.g., E1 to 2 box is the 10 bp surrounding E2).