Table 1.

Strains and plasmids used in this study

Strain or plasmidaGenotype or yeast markersReference
Strains
 MS1919 MAT a his3Δ200 ura3-52 ade2-101 trp1Δ1 This work
 MS2710 matΔ::LEU2 ura3-52 trp1Δ1 his3::TRP1 leu2-3,112 kar4-2150[MATα URA3 CEN ARS] 24
 MS3216 MAT a leu2-3,112 his3Δ200 ura3-52 ade2-101 kar4Δ::HIS3 25
 MS3212 MATα leu2-3,112 his3Δ200 ura3-52 ade2-101 kar4Δ::HIS3 25
 MY3371 MAT a ura3-52 leu2Δ1 This work
 MY3375 MAT a ura3-52 leu2Δ1 his3Δ200 This work
 MY4166 MAT a ura3-52 leu2Δ1 his3Δ200 kar4Δ::HIS3 This work
 MY4239 MATα ura3-52 leu2Δ1 his3Δ200 kar4Δ::HIS3 This work
 MY5792 MAT a ura3-52 leu2Δ1 his3Δ200 KAR4::HA This work
Plasmids
 pMR2516 KAR4 CEN ARS URA3 25
 pMR2654 KAR4::HA CEN ARS URA3 25
 pMR2973 PGAL-KAR4-long CEN ARS URA3 This work
 pMR3291 PGAL-KAR4-short CEN ARS URA3 This work
 pMR3356 PGAL-KAR4::HA-short CEN ARS URA3 This work
 pMR3357 KAR4::HA(ATG1→AAG) CEN ARS URA3 This work
 pMR3359 KAR4::HA(ATG2→AAG) CEN ARS URA3 This work
 pMR3459 PGAL-KAR4::HA-long CEN ARS URA3 This work
 pRS416 CEN ARS URA3 40
  • a All strains are from the Rose laboratory and are isogenic with S288C (hap2 mal kss1). The MY yeast strains are GAL2, and the MS yeast strains aregal2.