TABLE 1.

Partial list of RUNX-1-associated proteins identified by whole-lane MS

ProteinNo. of proteins identified in the indicated exptsfTotal no. of peptidescTotal no. of peptides in controld
Expt 1aExpt 2aExpt 3bExpt 4bExpt 5b
RUNX-1672593191230
CBF-β322233421
GATA-11020031
PML206000260
PRMT-10043070
Sin3A0030030
FLI-1220531310
Total no. of proteins1834635824373
Total no. of specific proteinse1521421914322
  • a Tandem anti-FLAG-SA affinity purification.

  • b Single SA affinity purification.

  • c Experiment-to-experiment variation in the number of peptides obtained for each of the proteins likely reflects differences in the amount of input material used, purification strategy, and/or sampling and detection issues related to the LC−MS-MS analysis. The total number of peptides corresponding to each protein from five independent purifications is shown.

  • d From cells expressing birA alone.

  • e Proteins not identified in cells expressing birA alone.

  • f For each experiment, the total number of milligrams of input protein used was as follows: experiment 1, 288 mg; experiment 2, 120 mg; experiment 3, 170 mg; experiment 4, 55 mg; experiment 5, 100 mg.