Table 3.

Recovery of ATG mutants from G1 arrest

Plasmida% Budded cells atb:
0 min10 min20 min30 min40 min50 min60 min
Vector330561720
KAR4 13028293750
ATG1→AAG26438434256
ATG2→AAG21115205049
  • a A kar4Δ strain (MS3216) harbored the vector control (pRS416), the KAR4 wild-type construct (pMR2654), the ATG1→AAG mutant construct (pMR3357), or the ATG2→AAG mutant construct (pMR3359).

  • b The numbers below the time points are the percentages of budded cells at the times (min) after release from pheromone-induced G1 arrest.