TABLE 4.

Comparative analysis of p97/VCP particle size distribution in solution using DLS

ProteinPolydispersity index (arbitrary unit)Peak 1Peak 2
Diam (nm)Intensity (%)Diam(nm)Intensity (%)
−ATP
    p97WT0.380 ± 0.0422.73 ± 1.8984.1 ± 2.5413.5 ± 92.515.9 ± 2.5
    p97R155P0.433 ± 0.0321.80 ± 0.3781.9 ± 2.7384.1 ± 99.918.1 ± 2.7
    p97A232E0.453 ± 0.0123.47 ± 0.4380.8 ± 0.7345.1 ± 21.819.1 ± 0.8
    p97K524A0.329 ± 0.0230.75 ± 1.49a95.8 ± 4.2a734.0 ± 148a4.20 ± 1.1a
+ATP
    p97WT0.343 ± 0.01325.25 ± 0.9787.4 ± 1.9311.4 ± 116.212.5 ± 1.9
    p97R155P0.459 ± 0.12222.13 ± 0.70a71.1 ± 1.7a236.5 ± 26.0628.8 ± 2.1a
    p97A232E0.954 ± 0.080a20.11 ± 0.12a35.0 ± 2.0a239.5 ± 16.6565.0 ± 2.0a
    p97K524A0.333 ± 0.01334.55 ± 0.66a87.2 ± 1.1403.3 ± 130.012.8 ± 1.1
  • a Statistically significant from p97WT at P ≤ 0.05 (Scheffe).