Construct | No. of: | % Sporulation | n | |||
---|---|---|---|---|---|---|

Single cells | Dyads | Triads | Tetrads | |||

Vector | 800 | 0 | 0 | 0 | 0 | 800 |

KAR4
| 571 | 39 | 32 | 52 | 18 | 694 |

ATG_{1}→AAG | 790 | 35 | 35 | 30 | 11 | 890 |

ATG_{2}→AAG | 675 | 30 | 32 | 40 | 13 | 777 |

↵a A

*kar4Δ/kar4Δ*diploid (MS3216 × MY4239) harbored the vector control (pRS416), the*KAR4*wild-type construct (pMR2654), the ATG_{1}→AAG mutant construct (pMR3357), or the ATG_{2}→AAG mutant construct (pMR3359). All strains were sporulated on plates for 5 days. The single-cell category included all cells with one nucleus as well as monad sporulation products. The dyad category refers to asci with two spores, the triad category refers to those with three spores, and the tetrad category refers to those with four spores. For each construct, % sporulation was calculated as the sum of the dyad, triad, and tetrad categories divided by the sample size (n) multiplied by 100.