TABLE 1.

Quantification of p97/VCP ATPase activity

ProteinActivity (nmol Pi μg−1 min−1)
37°C42°C
p97WT0.0289 ± 0.00650.0412 ± 0.0048
p97R155P0.0885 ± 0.0098a0.1471 ± 0.0309a,b
p97A232E0.0742 ± 0.0058a0.1036 ± 0.0156a
p97K524A0.0016 ± 0.0024a0.0010 ± 0.0017a
  • a Statistically significant compared to p97WT (Tukey/Kramer test; P ≤ 0.05).

  • b Statistically significant compared to p97A232E (Tukey/Kramer test; P ≤ 0.05).