Table 4.

Cell cycle distribution of cultures overexpressing Kar4p

PlasmidaTime (h) after induction% of cells that wereb:
Unbudded (G1)Small buddedMedium buddedLarge budded, single nucleus (G2/M)Large budded, two nuclei
KAR4 (wild type)03794167
PGAL-KAR4-short039838411
PGAL-KAR4-long0371133415
KAR4 (wild type)646731511
PGAL-KAR4-short655218169
PGAL-KAR4-long6556131313
  • a A wild-type strain (MY3375) carried plasmids which were expressing wild-type levels of Kar4p (pMR2654) or overexpressing Kar4p-long (pMR3459) or Kar4p-short (pMR3356).

  • b For the 0-h time point, 300 cells were counted for each sample. For the 6-h time point, 500 cells were counted for each sample.