TABLE 2.

Effects of telomere size (at the time of senescence) on telomeric recombination on solid medium in telomerase-negative (tlc1Δ) mutantsa

Survivor fate and type of telomeric recombination initiated in the presence of wild-type-length telomeresbRelevant genotypeSurvivor fate and type of telomeric recombination initiated in the presence of overelongated telomeresc
I or IItlc1ΔII then Id
Itlc1Δ rad50ΔI
IItlc1Δ rad51ΔII
Deathtlc1Δ rad52ΔII
Itlc1Δ rad59ΔI
Itlc1Δ rfa1-t11II
Deathtlc1Δ rad50Δ rad51ΔII
Itlc1Δ rad50Δ rad59ΔI
NDetlc1Δ rad50Δ rfa1-t11Death (6)
Atypical Itlc1Δ rad51Δ rfa1-t11II
Deathtlc1Δ rad59Δ rfa1-t11Death (2)
Deathtlc1Δ rad50Δ rad51Δ rad59ΔDeath (8)
Deathtlc1Δ rad50Δ rad51Δ rfa1-t11Death (15)
Deathtlc1Δ rad50Δ rad59Δ rfa1-t11Death (2)
  • a A RAD52+ background was used throughout except for the tlc1Δ rad52Δ strain (row 4). Restreak assays were performed.

  • b Data are from previous work from our laboratory (25, 26).

  • c Numbers in parentheses indicate the number of passages before death occurred (∼30 generations per passage).

  • d See Fig. 9.

  • e ND, not determined.