TABLE 1.

ColGalT activities measured in Sf9 cell lysatesa

Collagen usedTFMSbColGalT activity (pmol·min−1·mg protein−1)
Sf9cGLT25D1GLT25D2CEECAM1
None (no acceptor)1.5 ± 0.61.9 ± 0.70.9 ± 0.40.5 ± 0.1
Type I10.5 ± 1.843.7 ± 12.057.2 ± 8.58.7 ± 2.0
+5.0 ± 3.061.4 ± 18.218.0 ± 5.42.7 ± 1.9
Type II6.5 ± 0.836.6 ± 3.735.1 ± 3.83.3 ± 1.3
+3.4 ± 0.867.0 ± 16.522.1 ± 8.82.8 ± 0.8
Type III2.9 ± 0.931.7 ± 5.825.2 ± 6.81.8 ± 0.8
+11.0 ± 3.6244.7 ± 25.7102.1 ± 17.66.5 ± 1.7
Type IV1.7 ± 1.43.6 ± 2.23.6 ± 0.91.9 ± 0.7
+12.9 ± 4.6433.2 ± 49.6164.2 ± 43.38.4 ± 2.4
Type V2.3 ± 0.72.7 ± 0.32.3 ± 0.40.7 ± 0.1
+2.7 ± 1.618.6 ± 3.64.7 ± 1.52.0 ± 1.1
  • a Each value represents the average ± standard deviation for four assays.

  • b TFMS-mediated deglycosylation.

  • c Sf9 cells infected with an empty baculovirus were used.