Table 3.

Expression of amdS::lacZ fusions in the hapBΔ and hapEΔ backgrounds under induction with omega amino acids

StrainRelevant genotypeβ-Galactosidase activitya (U)
10 mMl-alanine10 mM l-alanine + 10 mM GABA10 mM l-alanine + 10 mM β-alanine
MH3408 amdS::lacZ 19.14 (2.02)94.24 (7.16)148.70 (3.56)
MH9207 amdS::lacZ; hapBΔ 1.95 (0.34)2.26 (0.55)1.10 (0.07)
MH9206 amdS::lacZ; hapEΔ 1.14 (0.08)1.21 (0.07)0.83 (0.07)
  • a Mycelia were grown in 100 ml of medium at 37°C for 16 h with 1% glucose. Nitrogen sources were added as indicated. Data represent the results of four separate experiments; standard errors are given in parentheses.