Table 1.

Effects of TPA on differentiation of TUR and HL-525 cell transfectantsa

Cell typeCell growth (% of control)Adherent cells (% of total cells)% of cells showing NSE staining
U-9373096>90
TUR/neo-1872<5
TUR/neo-2835<5
TUR/PKCβII-14993>75
TUR/PKCβII-24791>75
HL-602997>90
HL-525/neo-11013<5
HL-525/neo-2973<5
HL-525/PKCβII-13589>85
HL-525/PKCβII-24191>80
  • a Cells were seeded onto six-well tissue culture dishes (2 × 105 cells/well) and cultured in the presence or absence of 16 nM TPA for 48 h. Viability, as determined by trypan blue exclusion, was >95% for adherent and nonadherent cells. NSE staining was performed at 72 h. Results are expressed as the mean of three determinations (standard error, <10%).