Table 3.

Mapping of KSR1 sites phosphorylated by MAPK in vitro

HPLC fraction(s)Edman degradationbPhosphoamino acid(s)Amino acid residues spanned by deletion mutantSite identified (sequencea)
37–386T249–320T274 (LKPPRT*PPPPSR)
5311T249–320T260 (ALHSFITPPTT*PQLR)
59S, T424–539S443?
61–62S, T424–539S443?
65–66S, T249–320?
  • a Asterisks indicate sites of phosphorylation.

  • b Cycle in which 32P counts per minute were released.