Table 2.

Tryptic HPLC profiles of in vivo 32P-labeled KSR1 mutant proteins

      ConstructHPLC32P profile
WT KSR1 aloneContains major peaksa in fr.b 12 and 35
WT KSR1 + RasV12 Contains major peaks in fr. 12, 35, 37, 53, and 60–62
S297A-KSR1 aloneMissing peak in fr. 12
S392A-KSR1 aloneMissing peak in fr. 35
S297A,S392A-KSR1 aloneMissing peaks in fr. 12 and 35
S838A-KSR1 aloneContains major peaks in fr. 12 and 35
T274A-KSR1 + RasV12 Missing peak in fr. 37
S443A-KSR1 + RasV12 Missing peak in fr. 60–62
T260A,T274A,S443A-KSR1 + RasV12 Missing peak in fr. 37, 53, and 59–62
  • a Containing >500 cpm.

  • b fr., fraction.