TABLE 1.

Metastatic potential of Neu-expressing NMuMG-derived cell populations

NMuMG explantMetastasis results according to injection route
Mammary fat pada (tumors not removed)Mammary fat padb (tumors resected, 150-200 mm3)Tail veinc
% (no.) of mice with lung metastasesNo. of lesions/lungd% (no.) of mice with lung metastasesNo. of lesions/lungd% (no.) of mice with lung metastasesNo. of lesions/lungd
    Neu-NT100 (4/4)2-580 (4/5)2-11100 (4/4)8-18
    Neu-NYPD0 (0/4)025 (2/8)1-20 (0/5)0
  • a At the time of sacrifice (28 days), Neu-NT mammary tumors had reached volumes of 400 to 700 mm3, while all Neu-NYPD mammary tumors reached volumes of less than 100 mm3.

  • b Neu-NT mammary tumors were resected between 7 and 25 days postinjection, whereas Neu-NYPD tumors were resected between 38 and 67 days postinjection. Mice were sacrificed at 67 days postinjection.

  • c A total of 2 × 105 Neu-expressing cells were injected into the lateral tail vein. All mice were sacrificed at 8 weeks postinjection.

  • d A histological evaluation was performed on all five lobes of each lung and four step sections (100 μm between each step was analyzed).