TABLE 1.

Cell cycle distribution of cells expressing phosphorylation site mutant CK2α proteins 12 h after release from a thymidine block

Cell line and treatment% of cellsc
Sub-G1G1SG2aMb
CK2α-HA
    Plus tetracycline0.6432.0413.7748.794.01
    Minus tetracycline0.5929.8915.1649.604.13
CK2α-4D-HA
    Plus tetracycline 0.36 28.9117.97 46.79 4.85
    Minus tetracycline 7.92 33.2124.98 32.27 0.97
CK2α-4E-HA
    Plus tetracycline 0.44 31.7115.47 44.08 7.26
    Minus tetracycline 7.85 33.0522.77 34.40 1.23
CK2α-4A-HA
    Plus tetracycline0.4131.2216.3644.676.80
    Minus tetracycline0.3531.2915.4546.595.91
  • a Cells with 4N DNA content negative for phospho-histone H3 staining.

  • b Cells with 4N DNA content positive for phospho-histone H3 staining.

  • c Boldface indicates significant differences between samples with and without tetracycline (P < 0.01).