TABLE 2.

Cell cycle distribution of nocodazole-arrested cells expressing phosphorylation site mutant CK2α proteins

Cell line and treatment% of cellsa
Sub-G1G1SG2/M
CK2α-HA
    Plus tetracycline7.006.3310.5788.26
    Minus tetracycline7.016.0411.5073.50
CK2α-4D-HA
    Plus tetracycline3.893.1310.8871.80
    Minus tetracycline4.287.3317.0063.29
CK2α-4E-HA
    Plus tetracycline3.405.8111.4176.86
    Minus tetracycline4.928.9118.8473.59
CK2α-4A-HA
    Plus tetracycline 5.24 5.5712.37 82.64
    Minus tetracycline 14.87 10.9324.74 51.41
  • a Boldface indicates significant differences between samples with and without tetracycline (P < 0.01).