Table 4.

Tryptic HPLC profiles of in vitro 32P-labeled KSR1 mutant proteins

    ConstructHPLC32P profile
WT KSR1Contains major peaksa in fr.b37–38, 53, 59, 61–62, and 65–66
T260A-KSR1Missing peak in fr. 53
T274A-KSR1Missing peak in fr. 37–38
S443A-KSR1Missing peak in fr. 59, and 61–62
T274A,S443A-KSR1Missing peak in fr. 37–38, 59, and 61–62
T260A,S274A,S443A-KSR1Missing peak in fr. 37–38, 53, 59, and 61–62
  • a Containing >500 cpm.

  • b fr., fraction.