Table 6.

Kinds and locations of unequivocally independent point mutations induced in the lacZα gene during replication of UV-irradiated M13mp2-oriR by HeLa cell extractsa

MutantPosition(s)MutationPhotoproduct context (5′→3′)cStrandb
HR14−57C · G→T · AAGCTCACTCLeading
HR2558C · G→T · AAATTCACTGLeading
HR1558C · G→T · AAATTCACTGLeading
HR575C · G→T · ATTTACAACGLeading
HR988C · G→T · ATTTCCCAGTLagging
HR1188C · G→T · ATTTCCCAGTLagging
HR1989C · G→T · ATTTCCCAGTLagging
HR689C · G→T · ATTTCCCAGTLagging
HR2090C · G→T · ATTTCCCAGTLagging
HR790C · G→T · ATTTCCCAGTLagging
HR1090C · G→T · ATTTCCCAGTLagging
HR1390C · G→T · ATTTCCCAGTLagging
HR896C · G→T · AAAACCCTGGLeading
HR1296C · G→T · AAAACCCTGGLeading
HR24107–108C · G→T · A, C · G→T · ATTACCCAACLeading
HR16108C · G→T · ATACCCAACTLeading
HR26120C · G→T · ATCGCCTTGCLeading
HR23129C · G→T · AAGCACATCCLeading
HR2784C · G→A · TCCAGTCACGLagging
HR22115T · A→A · TGCGATTAAGLagging
HR17132–136ΔCCATCCCCCTLeading
  • a The target genes from 37 mutants derived with HeLa cell extracts were sequenced. Of these, 10 had no mutation in the target gene and one had a large deletion. Three had background mutations and were omitted from the analysis.

  • b Strand, as in Table 3.

  • c The site of the mutation is underlined.