TABLE 3.

Silencing phenotypes of N-terminal Sir3 BAH domain mutantsa

Sir3Silencing ability of indicated mutant at indicated locusExpression levelPhenotype
sir3Δ, telomeresir3Δ, HMLsir3Δ, HMRSir3Δ sir1Δ, HMLsir3Δ sir1Δ, HMR
WT++++++++++++++++++
T4F+++++++++Weak
L5A+++++++/−+++Weak
D7A+++++++++++++++NDNone
D7K+++++++++++++++NDNone
D7N+++++++++++++++NDNone
Vector
  • a Different levels of silencing are indicated as +++ to − based on comparison to wild type (+++) or vector (−) phenotypes. The strains used are those listed for Table 2.